Advances in Stem Cell Therapy by Nagwa El-Badri

Author:Nagwa El-Badri
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

8.8.6 mRNA and Protein

Messenger RNA (mRNA) and protein methods of reprogramming are nonintegrating and DNA-free methods of reprogramming. Modified mRNA encoding the reprogramming factors or proteins is transfected into cells to initiate the reprogramming process. Warren et al. showed that mRNA modified to improve translation, enhance RNA half-life, and escape a cell’s antiviral response to ssRNA can reprogram human fibroblasts to iPSCs [98]. Proteins must be engineered to contain a transduction domain, such as poly-arginine, to allow the protein to cross the cell membrane [99, 100]. Multiple, and often daily, cell transfections are required to successfully reprogram cells owing to the rapid turnover of mRNA and proteins. Although these methods can generate transgene-free and vector-free iPSC lines, the methods are labor intensive, and the reprogramming efficiency is often low compared to other reprogramming methods.

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.